1N2N

Crystal structure of cyanide complex of the oxygenase domain of inducible nitric oxide synthase.

1N2N の概要

• エントリーDOI: 10.2210/pdb1n2n/pdb
• 分子名称: Inducible Nitric Oxide Synthase, CYANIDE ION, ZINC ION, ... (7 entities in total)
• 機能のキーワード: nitric oxide synthase, cyanide complex, oxygen complex analogue, oxidoreductase
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99219.06

構造登録者

Fedorov, R.,Ghosh, D.K.,Schlichting, I. (登録日: 2002-10-23, 公開日: 2003-02-11, 最終更新日: 2023-10-25)

主引用文献

Fedorov, R.,Ghosh, D.K.,Schlichting, I.
Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase-structural models of the short-lived oxygen complexes
Arch.Biochem.Biophys., 409:25-31, 2003

PubMed Abstract: The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8A resolution and found to be identical with the structure of the active oxygen complex [I. Schlichting et al., 2000, Science 287, 1615]. Notably, cyanide binds in a bent mode and induces the active conformation that is characterized by the presence of two water molecules and a flip of the carbonyl of the conserved Asp251. The structure of the ternary complex of cyanide, L-arginine, and the oxygenase domain of inducible nitric oxide synthase was determined to 2.4A resolution. Cyanide binds essentially linearly, interacts with L-Arg, and induces the binding of a water molecule at the active site. This water is positioned by backbone interactions, located 2.8A from the nitrogen atom of cyanide, and could provide a proton required for O-O bond scission in the hydroxylation reaction of nitric oxide synthase.

PubMed: 12464241
DOI: 10.1016/S0003-9861(02)00555-6

実験手法

X-RAY DIFFRACTION (2.4 Å)