1MZI

Solution ensemble structures of HIV-1 gp41 2F5 mAb epitope

1MZI の概要

• エントリーDOI: 10.2210/pdb1mzi/pdb
• 分子名称: 2F5 epitope of HIV-1 gp41 fusion protein (1 entity in total)
• 機能のキーワード: ensemble, statistics, viral protein
• 細胞内の位置: Transmembrane protein gp41: Virion membrane; Single-pass type I membrane protein. Surface protein gp120: Virion membrane; Peripheral membrane protein: P04578
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1617.82

構造登録者

Barbato, G.,Bianchi, E.,Ingallinella, P.,Hurni, W.H.,Miller, M.D.,Ciliberto, G.,Cortese, R.,Bazzo, R.,Shiver, J.W.,Pessi, A. (登録日: 2002-10-08, 公開日: 2003-09-02, 最終更新日: 2024-05-29)

主引用文献

Barbato, G.,Bianchi, E.,Ingallinella, P.,Hurni, W.H.,Miller, M.D.,Ciliberto, G.,Cortese, R.,Bazzo, R.,Shiver, J.W.,Pessi, A.
Structural analysis of the epitope of the anti-HIV antibody 2F5 sheds light into its mechanism of neutralization and HIV fusion.
J.Mol.Biol., 330:1101-1115, 2003

PubMed Abstract: Inhibition of human immunodeficiency virus (HIV) fusion with the host cell has emerged as a viable therapeutic strategy, and rational design of inhibitors and vaccines, interfering with this process, is a prime target for antiviral research. To advance our knowledge of the structural biology of HIV fusion, we have studied the membrane-proximal region of the fusogenic envelope subunit gp41, which includes the epitope ELDKWA of the broadly neutralizing human antibody 2F5. The structural evidence available for this region is contradictory, with some studies suggesting an overall helical conformation, while the X-ray structure of the ELDKWAS peptide bound to the antibody shows it folded in a type I beta turn. We used a two-step strategy: Firstly, by a competition binding assay, we identified the proper boundaries of the domain recognized by 2F5, which we found considerably larger than the ELDKWAS hexapeptide. Secondly, we studied the structure of the resulting 13 amino acid residue peptide by collecting NMR data and analyzing them by our previously developed statistical method (NAMFIS). Our study revealed that the increase in binding affinity goes in parallel with stabilization of specific local and global conformational propensities, absent from the shorter epitope. When compounded with the available biological evidence, our structural analysis allows us to propose a specific role for the membrane-proximal region during HIV fusion, in terms of a conformational transition between the turn and the helical structure. At the same time, our hypothesis offers a structural explanation for the mechanism of neutralization of mAb 2F5.

PubMed: 12860131
DOI: 10.1016/S0022-2836(03)00611-9

実験手法

SOLUTION NMR