1MVM

MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C

1MVM の概要

• エントリーDOI: 10.2210/pdb1mvm/pdb
• 分子名称: PROTEIN (MURINE MINUTE VIRUS COAT PROTEIN), DNA (5'-D(*CP*CP*AP*CP*CP*CP*CP*AP*AP*CP*A)-3'), DNA (5'-D(*CP*AP*AP*A)-3'), ... (4 entities in total)
• 機能のキーワード: complex (viral coat protein-dna), viral coat protein/nucleic acid, icosahedral virus, virus-dna complex, virus/dna
• 由来する生物種: Minute virus of mice
• 細胞内の位置: Virion (By similarity): P07302
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 69492.81

構造登録者

Llamas-Saiz, A.L.,Agbandje-McKenna, M.,Rossmann, M.G. (登録日: 1996-06-21, 公開日: 1998-02-25, 最終更新日: 2024-04-03)

主引用文献

Llamas-Saiz, A.L.,Agbandje-McKenna, M.,Wikoff, W.R.,Bratton, J.,Tattersall, P.,Rossmann, M.G.
Structure determination of minute virus of mice.
Acta Crystallogr.,Sect.D, 53:93-102, 1997

PubMed Abstract: The three-dimensional crystal structure of the single-stranded DNA-containing ('full') parvovirus, minute virus of mice (MVM), has been determined to 3.5 A resolution. Both full and empty particles of MVM were crystallized in the monoclinic space group C2 with cell dimensions of a = 448.7, b = 416.7, c = 305.3 A and beta = 95.8 degrees. Diffraction data were collected at the Cornell High Energy Synchrotron Source using an oscillation camera. The crystals have a pseudo higher R32 space group in which the particles are situated at two special positions with 32 point symmetry, separated by (1/2)c in the hexagonal setting. The self-rotation function showed that the particles are rotated with respect to each other by 60 degrees around the pseudo threefold axis. Subsequently, a more detailed analysis of the structure amplitudes demonstrated that the correct space-group symmetry is C2 as given above. Only one of the three twofold axes perpendicular to the threefold axis in the pseudo R32 space group is a 'true' crystallographic twofold axis; the other two are only 'local' non-crystallographic symmetry axes. The known canine parvovirus (CPV) structure was used as a phasing model to initiate real-space electron-density averaging phase improvement. The electron density was easily interpretable and clearly showed the amino-acid differences between MVM and CPV, although the final overall correlation coefficient was only 0.63. The structure of MVM has a large amount of icosahedrally ordered DNA, amounting to 22% of the viral genome, which is significantly more than that found in CPV.

PubMed: 15299974
DOI: 10.1107/S0907444996010566

実験手法

X-RAY DIFFRACTION (3.5 Å)