1MSP

MAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6

1MSP の概要

• エントリーDOI: 10.2210/pdb1msp/pdb
• 分子名称: MAJOR SPERM PROTEIN (2 entities in total)
• 機能のキーワード: cytoskeletal protein, sperm, cell motility protein
• 由来する生物種: Ascaris suum (pig roundworm)
• 細胞内の位置: Cell projection, pseudopodium: P27439
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28556.21

構造登録者

Bullock, T.L.,Roberts, T.M.,Stewart, M. (登録日: 1996-05-20, 公開日: 1996-12-07, 最終更新日: 2024-02-14)

主引用文献

Bullock, T.L.,Roberts, T.M.,Stewart, M.
2.5 A resolution crystal structure of the motile major sperm protein (MSP) of Ascaris suum.
J.Mol.Biol., 263:284-296, 1996

PubMed Abstract: We have determined the structure of the Ascaris major sperm protein (MSP) to 2.5 A resolution using X-ray crystallography. The MSP polypeptide chain has an immunoglobulin-like fold based on a seven-stranded beta sandwich. In two strands, cis-proline residues impart distinctive kinks, and overall the structure most closely resembles that of the N-terminal domain of the bacterial chaperonin, PapD. In the C2 crystal form which we have solved here, two MSP chains are tightly associated in the asymmetric unit and are related by a non-crystallographic 2-fold rotation axis. This arrangement almost certainly represents the MSP dimer that is present in solution. Additionally, the arrangement of two MSP dimers at one of the crystallographic 2-fold axes in the 215 A unit cell suggests a possible mode for the assembly of MSP into the filaments which promote cell movement. This dimer-dimer association is based on a beta sheet extension mechanism between adjoining MSP monomers which resembles the interaction between PapD and its protein substrate.

PubMed: 8913307
DOI: 10.1006/jmbi.1996.0575

実験手法

X-RAY DIFFRACTION (2.5 Å)