1MSF

SOLUTION STRUCTURE OF A SPECIFIC DNA COMPLEX OF THE MYB DNA-BINDING DOMAIN WITH COOPERATIVE RECOGNITION HELICES

1MSF の概要

• エントリーDOI: 10.2210/pdb1msf/pdb
• 分子名称: DNA (5'-D(*CP*CP*TP*AP*AP*CP*TP*GP*AP*CP*AP*CP*AP*CP*AP*T)-3'), DNA (5'-D(*AP*TP*GP*TP*GP*TP*GP*TP*CP*AP*GP*TP*TP*AP*GP*G)-3'), C-Myb DNA-Binding Domain (3 entities in total)
• 機能のキーワード: dna binding protein/dna, dna binding protein-dna complex
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P06876
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 22472.17

構造登録者

Ogata, K.,Morikawa, S.,Nakamura, H.,Sekikawa, A.,Inoue, T.,Kanai, H.,Sarai, A.,Ishii, S.,Nishimura, Y. (登録日: 1995-01-24, 公開日: 1995-03-31, 最終更新日: 2024-05-22)

主引用文献

Ogata, K.,Morikawa, S.,Nakamura, H.,Sekikawa, A.,Inoue, T.,Kanai, H.,Sarai, A.,Ishii, S.,Nishimura, Y.
Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices.
Cell(Cambridge,Mass.), 79:639-648, 1994

PubMed Abstract: The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be a recognition helix. R2 and R3 are closely packed in the major groove, so that the two recognition helices contact each other directly to bind to the specific base sequence, AACNG cooperatively; this is a significant arrangement of recognition helices. The three key base pairs in this sequence are specifically recognized by Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no specific interactions with DNA from our NMR study of the DNA complex of the full DNA-binding domain (R1R2R3).

PubMed: 7954830
DOI: 10.1016/0092-8674(94)90549-5

実験手法

SOLUTION NMR