1MP1

Solution structure of the PWI motif from SRm160

1MP1 の概要

• エントリーDOI: 10.2210/pdb1mp1/pdb
• NMR情報: BMRB: 5162
• 分子名称: Ser/Arg-related nuclear matrix protein (1 entity in total)
• 機能のキーワード: four helix bundle, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus matrix: Q8IYB3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12869.05

構造登録者

Szymczyna, B.R.,Bowman, J.,McCracken, S.,Pineda-Lucena, A.,Lu, Y.,Cox, B.,Lambermon, M.,Graveley, B.R.,Arrowsmith, C.H.,Blencowe, B.J. (登録日: 2002-09-11, 公開日: 2003-09-16, 最終更新日: 2024-05-22)

主引用文献

Szymczyna, B.R.,Bowman, J.,McCracken, S.,Pineda-Lucena, A.,Lu, Y.,Cox, B.,Lambermon, M.,Graveley, B.R.,Arrowsmith, C.H.,Blencowe, B.J.
Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.
Genes Dev., 17:461-475, 2003

PubMed Abstract: The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.

PubMed: 12600940
DOI: 10.1101/gad.1060403

実験手法

SOLUTION NMR