1MNT

SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)

1MNT の概要

• エントリーDOI: 10.2210/pdb1mnt/pdb
• 分子名称: MNT REPRESSOR (1 entity in total)
• 機能のキーワード: transcription regulation
• 由来する生物種: Enterobacteria phage P22
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17652.00

構造登録者

Burgering, M.J.M.,Boelens, R.,Gilbert, D.E.,Breg, J.N.,Knight, K.L.,Sauer, R.T.,Kaptein, R. (登録日: 1994-06-28, 公開日: 1994-09-30, 最終更新日: 2024-05-22)

主引用文献

Burgering, M.J.,Boelens, R.,Gilbert, D.E.,Breg, J.N.,Knight, K.L.,Sauer, R.T.,Kaptein, R.
Solution structure of dimeric Mnt repressor (1-76).
Biochemistry, 33:15036-15045, 1994

PubMed Abstract: Wild-type Mnt repressor of Salmonella bacteriophage P22 is a tetrameric protein of 82 residues per monomer. A C-terminal deletion mutant of the repressor denoted Mnt (1-76) is a dimer in solution. The structure of this dimer has been determined using NMR. The NMR assignments of the majority of the 1H, 15N, and 13C resonances were obtained using 2D and triple-resonance 3D techniques. Elements of secondary structure were identified on the basis of characteristic sequential and medium range NOEs. For the structure determination more than 1000 NOEs per monomer were obtained, and structures were generated using distance geometry and restrained simulated annealing calculations. The discrimination of intra- vs intermonomer NOEs was based upon the observation of intersubunit NOEs in [15N,13C] double half-filtered NOESY experiments. The N-terminal part of Mnt (residues 1-44), which shows a 40% sequence homology with the Arc repressor, has a similar secondary and tertiary structure. Mnt (1-76) continues with a loop region of irregular structure, a third alpha-helix, and a random coil C-terminal peptide. Analysis of the secondary structure NOEs, the exchange rates, and the backbone chemical shifts suggests that the carboxy-terminal third helix is less stable than the remainder of the protein, but the observation of intersubunit NOEs for this part of the protein enables the positioning of this helix. The rsmd's between the backbone atoms of the N-terminal part of the Mnt repressor (residues 5-43, 5'-43') and the Arc repressor is 1.58 A, and between this region and the corresponding part of the MetJ repressor 1.43 A.

PubMed: 7999761
DOI: 10.1021/bi00254a012

実験手法

SOLUTION NMR