1MN7

NDP kinase mutant (H122G;N119S;F64W) in complex with aBAZTTP

1MN7 の概要

• エントリーDOI: 10.2210/pdb1mn7/pdb
• 関連するPDBエントリー: 1f6t 1lwx
• 分子名称: NDP kinase, MAGNESIUM ION, 3'-AZIDO-3'-DEOXY-THYMIDINE-5'-ALPHA BORANO TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ndp kinase-abazttp complex, transferase
• 由来する生物種: Dictyostelium discoideum
• 細胞内の位置: Cytoplasm: P22887
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34549.12

構造登録者

gallois-montbrun, s.,schneider, b.,chen, y.,giacomoni-fernandes, v.,mulard, l.,morera, s.,janin, j.,deville-bonne, d.,veron, m. (登録日: 2002-09-05, 公開日: 2002-10-02, 最終更新日: 2024-05-29)

主引用文献

gallois-montbrun, s.,schneider, b.,chen, y.,giacomoni-fernandes, v.,mulard, l.,morera, s.,janin, j.,deville-bonne, d.,veron, m.
Improving nucleoside diphosphate kinase for antiviral nucleotide analogs activation
J.BIOL.CHEM., 277:39953-39959, 2002

PubMed Abstract: Antiviral nucleoside analog therapies rely on their incorporation by viral DNA polymerases/reverse transcriptase leading to chain termination. The analogs (3'-deoxy-3'-azidothymidine (AZT), 2',3'-didehydro-2',3'-dideoxythymidine (d4T), and other dideoxynucleosides) are sequentially converted into triphosphate by cellular kinases of the nucleoside salvage pathway and are often poor substrates of these enzymes. Nucleoside diphosphate (NDP) kinase phosphorylates the diphosphate derivatives of the analogs with an efficiency some 10(4) lower than for its natural substrates. Kinetic and structural studies of Dictyostelium and human NDP kinases show that the sugar 3'-OH, absent from all antiviral analogs, is required for catalysis. To improve the catalytic efficiency of NDP kinase on the analogs, we engineered several mutants with a protein OH group replacing the sugar 3'-OH. The substitution of Asn-115 in Ser and Leu-55 in His results in an NDP kinase mutant with an enhanced ability to phosphorylate antiviral derivatives. Transfection of the mutant enzyme in Escherichia coli results in an increased sensitivity to AZT. An x-ray structure at 2.15-A resolution of the Dictyostelium enzyme bearing the serine substitution in complex with the R(p)-alpha-borano-triphosphate derivative of AZT shows that the enhanced activity reflects an improved geometry of binding and a favorable interaction of the 3'-azido group with the engineered serine.

PubMed: 12171931
DOI: 10.1074/jbc.M206360200

実験手法

X-RAY DIFFRACTION (2.15 Å)