1MMO

CRYSTAL STRUCTURE OF A BACTERIAL NON-HAEM IRON HYDROXYLASE THAT CATALYSES THE BIOLOGICAL OXIDATION OF METHANE

1MMO の概要

• エントリーDOI: 10.2210/pdb1mmo/pdb
• 分子名称: METHANE MONOOXYGENASE HYDROLASE (BETA CHAIN), METHANE MONOOXYGENASE HYDROLASE (ALPHA CHAIN), METHANE MONOOXYGENASE HYDROLASE (GAMMA CHAIN), ... (6 entities in total)
• 機能のキーワード: oxidoreductase (monooxygenase)
• 由来する生物種: Methylococcus capsulatus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 245693.23

構造登録者

Rosenzweig, A.C.,Frederick, C.A.,Lippard, S.J.,Nordlund, P. (登録日: 1994-02-22, 公開日: 1995-02-27, 最終更新日: 2024-02-14)

主引用文献

Rosenzweig, A.C.,Frederick, C.A.,Lippard, S.J.,Nordlund, P.
Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane.
Nature, 366:537-543, 1993

PubMed Abstract: The 2.2 A crystal structure of the 251K alpha 2 beta 2 gamma 2 dimeric hydroxylase protein of methane monooxygenase from Methylococcus capsulatus (Bath) reveals the geometry of the catalytic di-iron core. The two iron atoms are bridged by exogenous hydroxide and acetate ligands and further coordinated by four glutamate residues, two histidine residues and a water molecule. The dinuclear iron centre lies in a hydrophobic active-site cavity for binding methane. An extended canyon runs between alpha beta pairs, which have many long alpha-helices, for possible docking of the reductase and coupling proteins required for catalysis.

PubMed: 8255292
DOI: 10.1038/366537a0

実験手法

X-RAY DIFFRACTION (2.2 Å)