1MJ7

Crystal Structure Of The Complex Of The Fab fragment of Esterolytic Antibody MS5-393 and A Transition-State Analog

Summary for 1MJ7

• Entry DOI: 10.2210/pdb1mj7/pdb
• Related: 1MH5 1MIE 1MJ8 1MJJ 1MJU
• Descriptor: IMMUNOGLOBULIN MS5-393, N-{[2-({[1-(4-CARBOXYBUTANOYL)AMINO]-2-PHENYLETHYL}-HYDROXYPHOSPHINYL)OXY]ACETYL}-2-PHENYLETHYLAMINE, ... (4 entities in total)
• Functional Keywords: catalytic antibody, ester hydrolysis, esterolytic, fab, immunoglobulin, tsa, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 49214.85

Authors

Ruzheinikov, S.N.,Muranova, T.A.,Sedelnikova, S.E.,Partridge, L.J.,Blackburn, G.M.,Murray, I.A.,Kakinuma, H.,Takashi, N.,Shimazaki, K.,Sun, J.,Nishi, Y.,Rice, D.W. (deposition date: 2002-08-27, release date: 2003-09-23, Last modification date: 2024-11-20)

Primary citation

Ruzheinikov, S.N.,Muranova, T.A.,Sedelnikova, S.E.,Partridge, L.J.,Blackburn, G.M.,Murray, I.A.,Kakinuma, H.,Takashi, N.,Shimazaki, K.,Sun, J.,Nishi, Y.,Rice, D.W.
High-resolution crystal structure of the Fab-fragments of a family of mouse catalytic antibodies with esterase activity
J.Mol.Biol., 332:423-435, 2003

PubMed Abstract: The crystal structures of four related Fab fragments of a family of catalytic antibodies displaying differential levels of esterase activity have been solved in the presence and in the absence of the transition-state analogue (TSA) that was used to elicit the immune response. The electron density maps show that the TSA conformation is essentially identical, with limited changes on hapten binding. Interactions with the TSA explain the specificity for the D rather than the L-isomer of the substrate. Differences in the residues in the hapten-binding pocket, which increase hydrophobicity, appear to correlate with an increase in the affinity of the antibodies for their substrate. Analysis of the structures at the active site reveals a network of conserved hydrogen bond contacts between the TSA and the antibodies, and points to a critical role of two conserved residues, HisL91 and LysH95, in catalysis. However, these two key residues are set into very different contexts in their respective structures, with an apparent direct correlation between the catalytic power of the antibodies and the complexity of their interactions with the rest of the protein. This suggests that the catalytic efficiency may be controlled by contacts arising from a second sphere of residues at the periphery of the active site.

PubMed: 12948492
DOI: 10.1016/S0022-2836(03)00902-1

Experimental method

X-RAY DIFFRACTION (2.25 Å)