1MGX

COAGULATION FACTOR, MG(II), NMR, 7 STRUCTURES (BACKBONE ATOMS ONLY)

1MGX の概要

• エントリーDOI: 10.2210/pdb1mgx/pdb
• 分子名称: COAGULATION FACTOR IX (1 entity in total)
• 機能のキーワード: coagulation factor, plasma
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted : P00740
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6293.37

構造登録者

Freedman, S.J.,Furie, B.C.,Furie, B.,Baleja, J.D. (登録日: 1995-06-21, 公開日: 1996-11-08, 最終更新日: 2025-03-26)

主引用文献

Freedman, S.J.,Blostein, M.D.,Baleja, J.D.,Jacobs, M.,Furie, B.C.,Furie, B.
Identification of the phospholipid binding site in the vitamin K-dependent blood coagulation protein factor IX.
J.Biol.Chem., 271:16227-16236, 1996

PubMed Abstract: The blood coagulation and regulatory proteins that contain gamma-carboxyglutamic acid are a part of a unique class of membrane binding proteins that require calcium for their interaction with cell membranes. Following protein biosynthesis, glutamic acids on these proteins are converted to gamma-carboxyglutamic acid (Gla) in a reaction that requires vitamin K as a cofactor. The vitamin K-dependent proteins undergo a conformational transition upon metal ion binding, but only calcium ions mediate protein-phospholipid interaction. To identify the site on Factor IX that is required for phospholipid binding, we have determined the three-dimensional structure of the Factor IX Gla domain bound to magnesium ions by NMR spectroscopy. By comparison of this structure to that of the Gla domain bound to calcium ions, we localize the membrane binding site to a highly ordered structure including residues 1-11 of the Gla domain. In the presence of Ca2+, Factor IX Gla domain peptides that contain the photoactivatable amino acid p-benzoyl-L-phenylalanine at positions 6 or 9 cross-link to phospholipid following irradiation, while peptides lacking this amino acid analog or with this analog at position 46 did not cross-link. These results indicate that the NH2 terminus of the Gla domain, specifically including leucine 6 and phenylalanine 9 in the hydrophobic patch, is the contact surface on Factor IX that interacts with the phospholipid bilayer.

PubMed: 8663165
DOI: 10.1074/jbc.271.27.16227

実験手法

SOLUTION NMR