1MF4

Structure-based design of potent and selective inhibitors of phospholipase A2: Crystal structure of the complex formed between phosholipase A2 from Naja Naja sagittifera and a designed peptide inhibitor at 1.9 A resolution

1MF4 の概要

• エントリーDOI: 10.2210/pdb1mf4/pdb
• 関連するPDBエントリー: 1LFF 1LN8
• 分子名称: Phospholipase A2, VAL-ALA-PHE-ARG-SER, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: naja naja sagittifera, phospholipase a2, designed inhibitor, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Naja sagittifera; 詳細
• 細胞内の位置: Secreted: P60045
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13748.26

構造登録者

Singh, R.K.,Vikram, P.,Paramsivam, M.,Jabeen, T.,Sharma, S.,Makker, J.,Dey, S.,Kaur, P.,Srinivasan, A.,Singh, T.P. (登録日: 2002-08-09, 公開日: 2003-09-30, 最終更新日: 2024-11-13)

主引用文献

Singh, R.K.,Vikram, P.,Makker, J.,Jabeen, T.,Sharma, S.,Dey, S.,Kaur, P.,Srinivasan, A.,Singh, T.P.
Design of specific peptide inhibitors for group I phospholipase A2: structure of a complex formed between phospholipase A2 from Naja naja sagittifera (group I) and a designed peptide inhibitor Val-Ala-Phe-Arg-Ser (VAFRS) at 1.9 A resolution reveals unique features
Biochemistry, 42:11701-11706, 2003

PubMed Abstract: Phospholipase A(2) (PLA(2)) (E. C. 3.1.1.4) is a common enzyme in the two-way cascade mechanism leading to the production of proinflammatory compounds known as eicosanoids. The binding of phospholipase A(2) to the membrane surface and hydrolysis of phospholipids are thought to involve the formation of a hydrophobic channel into which a single substrate molecule diffuses before its cleavage. To regulate the production of proinflammatory compounds, a specific peptide inhibitor Val-Ala-Phe-Arg-Ser (VAFRS) for the group I PLA(2) enzymes has been designed and synthesized. PLA(2) was isolated from Indian cobra (Naja naja sagittifera) venom and purified to homogeneity. The binding studies indicated the K(i) value of 1.02 +/- 0.10 x 10(-8) M. The purified PLA(2) samples and the designed inhibitor VAFRS were cocrystallized. The crystal structure of the complex was determined and refined to 1.9 A resolution. The peptide binds to PLA(2) at the active site and fills the hydrophobic channel completely. However, its placement with respect to the channel is in the opposite direction as compared to those observed in group II PLA(2)'s. Furthermore, the predominant intermolecular interactions involve strong electrostatic interactions between the side chains of peptide Arg and Asp 49 of PLA(2) together with a number of van der Waals interactions with other residues. A good number of observed interactions between the peptide and the protein indicate the significance of a structure-based drug design approach. The novel factor in the present sequence of the peptide is related to the introduction of a positively charged residue at the C-terminal part of the peptide.

PubMed: 14529280
DOI: 10.1021/bi035076x

実験手法

X-RAY DIFFRACTION (1.9 Å)