1MBK

MOUSE C-MYB DNA-BINDING DOMAIN REPEAT 3

1MBK の概要

• エントリーDOI: 10.2210/pdb1mbk/pdb
• 分子名称: MYB PROTO-ONCOGENE PROTEIN (1 entity in total)
• 機能のキーワード: protooncogene product, dna binding protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P06876
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6259.19

構造登録者

Ogata, K.,Morikawa, S.,Nakamura, H.,Hojo, H.,Yoshimura, S.,Zhang, R.,Aimoto, S.,Ametani, Y.,Hirata, Z.,Sarai, A.,Ishii, S.,Nishimura, Y. (登録日: 1995-05-19, 公開日: 1995-07-31, 最終更新日: 2024-06-05)

主引用文献

Ogata, K.,Morikawa, S.,Nakamura, H.,Hojo, H.,Yoshimura, S.,Zhang, R.,Aimoto, S.,Ametani, Y.,Hirata, Z.,Sarai, A.,Ishii, S.,Nishimura, Y.
Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb.
Nat.Struct.Biol., 2:309-320, 1995

PubMed Abstract: The DNA-binding domain of c-Myb consists of three imperfect tandem repeats (R1, R2 and R3). The three repeats have similar overall architectures, each containing a helix-turn-helix variation motif. The three conserved tryptophans in each repeat participate in forming a hydrophobic core. Comparison of the three repeat structures indicated that cavities are found in the hydrophobic core of R2, which is thermally unstable. On complexation with DNA, the orientations of R2 and R3 are fixed by tight binding and their conformations are slightly changed. No significant changes occur in the chemical shifts of R1 consistent with its loose interaction with DNA.

PubMed: 7796266
DOI: 10.1038/nsb0495-309

実験手法

SOLUTION NMR