1M9O

NMR structure of the first Zinc Binding domain of Nup475/TTP/TIS11

1M9O の概要

• エントリーDOI: 10.2210/pdb1m9o/pdb
• NMR情報: BMRB: 5525
• 分子名称: Tristetraproline, ZINC ION (2 entities in total)
• 機能のキーワード: cys3his type zinc finger, metal binding protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P22893
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9037.60

構造登録者

Amann, B.T.,Worthington, M.T.,Berg, J.M. (登録日: 2002-07-29, 公開日: 2003-06-03, 最終更新日: 2024-05-22)

主引用文献

Amann, B.T.,Worthington, M.T.,Berg, J.M.
A Cys3His Zinc-Binding Domain from Nup475/Tristetraproline: a Novel Fold with a Disklike Structure
Biochemistry, 42:217-221, 2003

PubMed Abstract: Nup475 (also known as tristetraprolin and TIS11) includes two zinc-binding domains of the form Cys-X8-Cys-X5-Cys-X3-His. These domains are required for rapid degradation of tumor necrosis factor (TNF) and other mRNAs through the interaction with AU-rich elements in their 3'-untranslated regions. The three-dimensional solution structure of the first domain was determined by multidimensional nuclear magnetic resonance spectroscopy, revealing a novel fold around a central zinc ion. The core structure is disk-like with a diameter of approximately 25 A and a width of approximately 12 A. This structure provides a basis for evaluating the role of individual residues for structural stability and for nucleic acid binding.

PubMed: 12515557
DOI: 10.1021/bi026988m

実験手法

SOLUTION NMR