1M9G
Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI
Summary for 1M9G
| Entry DOI | 10.2210/pdb1m9g/pdb |
| NMR Information | BMRB: 5529 |
| Descriptor | Monellin chain B and Monellin chain A (1 entity in total) |
| Functional Keywords | 5 stranded beta sheet 1 helix, plant protein |
| Biological source | Dioscoreophyllum cumminsii (serendipity berry) |
| Total number of polymer chains | 1 |
| Total formula weight | 11433.08 |
| Authors | Spadaccini, R.,Trabucco, F.,Saviano, G.,Picone, D.,Crescenzi, O.,Tancredi, T.,Temussi, P.A. (deposition date: 2002-07-29, release date: 2003-06-10, Last modification date: 2024-05-29) |
| Primary citation | Spadaccini, R.,Trabucco, F.,Saviano, G.,Picone, D.,Crescenzi, O.,Tancredi, T.,Temussi, P.A. The Mechanism of Interaction of Sweet Proteins with the T1R2-T1R3 Receptor: Evidence from the Solution Structure of G16A-MNEI J.MOL.BIOL., 328:683-692, 2003 Cited by PubMed Abstract: The mechanism by which sweet proteins elicit a response on the T1R2-T1R3 sweet taste receptor is still mostly unknown but has been so far related to the presence of "sweet fingers" on the protein surface able to interact with the same mechanism as that of low molecular mass sweeteners. In the search for the identification of sweet fingers, we have solved the solution structure of G16A MNEI, a structural mutant that shows a reduction of one order of magnitude in sweetness with respect to its parent protein, MNEI, a single-chain monellin. Comparison of the structures of wild-type monellin and its G16A mutant shows that the mutation does not affect the structure of potential glucophores but produces a distortion of the surface owing to the partial relative displacement of elements of secondary structure. These results show conclusively that sweet proteins do not possess a sweet finger and strongly support the hypothesis that the mechanism of interaction of sweet-tasting proteins with the recently identified T1R2-T1R3 GPC receptor is different from that of low molecular mass sweeteners. PubMed: 12706725DOI: 10.1016/S0022-2836(03)00346-2 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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