1M8A

Human MIP-3alpha/CCL20

1M8A の概要

• エントリーDOI: 10.2210/pdb1m8a/pdb
• 関連するPDBエントリー: 1ha6
• 分子名称: Small inducible cytokine A20, ISOPROPYL ALCOHOL (3 entities in total)
• 機能のキーワード: cc-chemokine, il-8 type dimer, cytokine
• 細胞内の位置: Secreted: P78556
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16688.09

構造登録者

Hoover, D.M.,Boulegue, C.,Yang, D.,Oppenheim, J.J.,Tucker, K.,Lu, W.,Lubkowski, J. (登録日: 2002-07-24, 公開日: 2002-07-31, 最終更新日: 2024-10-30)

主引用文献

Hoover, D.M.,Boulegue, C.,Yang, D.,Oppenheim, J.J.,Tucker, K.,Lu, W.,Lubkowski, J.
The structure of human macrophage inflammatory protein-3alpha /CCL20. Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins
J.Biol.Chem., 277:37647-37654, 2002

PubMed Abstract: Human macrophage inflammatory protein-3alpha (MIP-3alpha; CCL20) is a CC-type chemokine that binds to and activates CC chemokine receptor-6 (CCR6). Although MIP-3alpha does not share the binding site of CCR6 with any other chemokine, human beta-defensin-1 and -2, small cationic antimicrobial peptides, have also been found to bind to and activate CCR6. Conversely, we have found that MIP-3alpha possesses antibacterial activity of greater potency than human beta-defensin-1 and -2 against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213, while having no activity against the fungus Candida albicans. There is no clear sequence similarity between beta-defensins and the chemokine MIP-3alpha, beyond an abundance of cationic residues and the presence of disulfide bonds. Nonetheless, there are structural similarities between these three proteins that allow their overlap of chemotactic and antimicrobial activities. In this report, we describe the x-ray crystal structure of human MIP-3alpha refined to a resolution of 1.7 A and compare it with the crystal structures of human beta-defensin-1 and -2. Molecules of MIP-3alpha and the beta-defensins seem to share few structural motifs that are likely associated with their common biological activities.

PubMed: 12149255
DOI: 10.1074/jbc.M203907200

実験手法

X-RAY DIFFRACTION (1.7 Å)