1M7Y

Crystal structure of apple ACC synthase in complex with L-aminoethoxyvinylglycine

1M7Y の概要

• エントリーDOI: 10.2210/pdb1m7y/pdb
• 関連するPDBエントリー: 1b8g 1m4n
• 分子名称: 1-aminocyclopropane-1-carboxylate synthase, (2E,3E)-4-(2-aminoethoxy)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid, (4R)-2-METHYLPENTANE-2,4-DIOL, ... (4 entities in total)
• 機能のキーワード: fruit ripening, ethylene biosynthesis, pyridoxal phosphate, lyase
• 由来する生物種: Malus x domestica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49599.27

構造登録者

Capitani, G.,McCarthy, D.,Gut, H.,Gruetter, M.G.,Kirsch, J.F. (登録日: 2002-07-23, 公開日: 2002-12-23, 最終更新日: 2023-10-25)

主引用文献

Capitani, G.,McCarthy, D.L.,Gut, H.,Gruetter, M.G.,Kirsch, J.F.
Apple 1-Aminocyclopropane-1-carboxylate Synthase in Complex with the Inhibitor L-Aminoethoxyvinylglycine
J.Biol.Chem., 277:49735-49742, 2002

PubMed Abstract: The 1.6-A crystal structure of the covalent ketimine complex of apple 1-aminocyclopropane-1-carboxylate (ACC) synthase with the potent inhibitor l-aminoethoxyvinylglycine (AVG) is described. ACC synthase catalyzes the committed step in the biosynthesis of ethylene, a plant hormone that is responsible for the initiation of fruit ripening and for regulating many other developmental processes. AVG is widely used in plant physiology studies to inhibit the activity of ACC synthase. The structural assignment is supported by the fact that the complex absorbs maximally at 341 nm. These results are not in accord with the recently reported crystal structure of the tomato ACC synthase AVG complex, which claims that the inhibitor only associates noncovalently. The rate constant for the association of AVG with apple ACC synthase was determined by stopped-flow spectrophotometry (2.1 x 10(5) m(-1) s(-1)) and by the rate of loss of enzyme activity (1.1 x 10(5) m(-1) s(-1)). The dissociation rate constant determined by activity recovery is 2.4 x 10(-6) s(-1). Thus, the calculated K(d) value is 10-20 pm.

PubMed: 12228256
DOI: 10.1074/jbc.M208427200

実験手法

X-RAY DIFFRACTION (1.6 Å)