1M7U

Crystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast

1M7U の概要

• エントリーDOI: 10.2210/pdb1m7u/pdb
• 関連するPDBエントリー: 1M6U
• 分子名称: Ndt80 protein (2 entities in total)
• 機能のキーワード: transcription activator, yeast protein, dna-binding, meiosis
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P38830
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62614.91

構造登録者

Montano, S.P.,Cote, M.L.,Fingerman, I.,Pierce, M.,Vershon, A.K.,Georgiadis, M.M. (登録日: 2002-07-22, 公開日: 2002-11-06, 最終更新日: 2024-02-14)

主引用文献

Montano, S.P.,Cote, M.L.,Fingerman, I.,Pierce, M.,Vershon, A.K.,Georgiadis, M.M.
Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast
Proc.Natl.Acad.Sci.USA, 99:14041-14046, 2002

PubMed Abstract: Ndt80 is a transcriptional activator required for meiosis in the yeast Saccharomyces cerevisiae. Here, we report the crystal structure at 2.3 A resolution of the DNA-binding domain of Ndt80 experimentally phased by using the anomalous and isomorphous signal from a single ordered Se atom per molecule of 272-aa residues. The structure reveals a single approximately 32-kDa domain with a distinct fold comprising a beta-sandwich core elaborated with seven additional beta-sheets and three short alpha-helices. Inspired by the structure, we have performed a mutational analysis and defined a DNA-binding motif in this domain. The DNA-binding domain of Ndt80 is homologous to a number of proteins from higher eukaryotes, and the residues that we have shown are required for DNA binding by Ndt80 are highly conserved among this group of proteins. These results suggest that Ndt80 is the defining member of a previously uncharacterized family of transcription factors, including the human protein (C11orf9), which has been shown to be highly expressed in invasive or metastatic tumor cells.

PubMed: 12384578
DOI: 10.1073/pnas.222312199

実験手法

X-RAY DIFFRACTION (2.8 Å)