1M7B

Crystal structure of Rnd3/RhoE: functional implications

Summary for 1M7B

• Entry DOI: 10.2210/pdb1m7b/pdb
• Descriptor: Rnd3/RhoE small GTP-binding protein, MAGNESIUM ION, GUANOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: small gtpase, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Golgi apparatus membrane; Peripheral membrane protein: P61587
• Total number of polymer chains: 1
• Total formula weight: 21039.59

Authors

Fiegen, D.,Blumenstein, L.,Stege, P.,Vetter, I.R.,Ahmadian, M.R. (deposition date: 2002-07-19, release date: 2002-08-07, Last modification date: 2023-10-25)

Primary citation

Fiegen, D.,Blumenstein, L.,Stege, P.,Vetter, I.R.,Ahmadian, M.R.
Crystal structure of Rnd3/RhoE: functional implications
FEBS LETT., 525:100-104, 2002

PubMed Abstract: The Rnd proteins constitute an exceptional subfamily within the Rho GTPase family. They possess extended chains at both termini and four prominent amino acid deviations causing GTPase deficiency. Herein, we report the crystal structure of the Rnd3/RhoE G-domain (amino acids 19-200) at 2.0 A resolution. This is the first GTP-structure of a Rho family member which reveals a similar fold but striking differences from RhoA concerning (i) GTPase center, (ii) charge distribution at several surface areas, (iii) C3-transferase binding site and (iv) interacting interfaces towards RhoA regulators and effectors.

PubMed: 12163169
DOI: 10.1016/S0014-5793(02)03094-6

Experimental method

X-RAY DIFFRACTION (2 Å)