1M6R

Crystal structure of rGd(CGCGCG) forming hexamer Z-DNA duplex with 5'-(rG) overhang

Summary for 1M6R

• Entry DOI: 10.2210/pdb1m6r/pdb
• Descriptor: 5'-R(*G)D(*CP*GP*CP*GP*CP*G)-3' (2 entities in total)
• Functional Keywords: z-dna, overhang, dna
• Total number of polymer chains: 2
• Total formula weight: 4278.82

Authors

Pan, B.,Sundaralingam, M. (deposition date: 2002-07-17, release date: 2003-02-25, Last modification date: 2024-02-14)

Primary citation

Pan, B.,Sundaralingam, M.
Crystal structure of rGd(CGCGCG): a Z-DNA hexamer duplex with a 5'-(rG) overhang.
Acta Crystallogr.,Sect.D, 59:433-437, 2003

PubMed Abstract: The crystal structure of the heptamer rGd(CGCGCG) has been determined at 1.54 A resolution with R(work) and R(free) of 0.191 and 0.235, respectively. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 17.96, b = 31.47, c = 44.73 A and two independent strands in the asymmetric unit. The chimera forms a Z-DNA hexamer duplex d(CGCGCG)(2), with the 5'-overhang rG invisible in the density. The replacement of rG in the 5' terminus of d(GCGCGCG) changes the reverse Hoogsteen G.G base pairing of the 5'-overhang dG (Pan et al., 1997). However, the replacement does not change the helix from Z-form to A-form or the water-structure motifs in the Z-DNA double-helical structure.

PubMed: 12595699
DOI: 10.1107/S0907444902022655

Experimental method

X-RAY DIFFRACTION (1.54 Å)