1M4X
PBCV-1 virus capsid, quasi-atomic model
1M4X の概要
エントリーDOI | 10.2210/pdb1m4x/pdb |
関連するPDBエントリー | 1j5q 1m3y |
分子名称 | PBCV-1 virus capsid (1 entity in total) |
機能のキーワード | icosahedral virus capsid, beta barrel, icosahedral virus, virus |
由来する生物種 | Paramecium bursaria Chlorella virus 1 |
タンパク質・核酸の鎖数 | 3 |
化学式量合計 | 137204.67 |
構造登録者 | Nandhagopal, N.,Simpson, A.A.,Gurnon, J.R.,Yan, X.,Baker, T.S.,Graves, M.V.,Van Etten, J.L.,Rossmann, M.G. (登録日: 2002-07-05, 公開日: 2002-12-04, 最終更新日: 2024-02-14) |
主引用文献 | Nandhagopal, N.,Simpson, A.,Gurnon, J.R.,Yan, X.,Baker, T.S.,Graves, M.V.,Van Etten, J.L.,Rossmann, M.G. The Structure and Evolution of the Major Capsid Protein of a Large, Lipid containing, DNA virus. Proc.Natl.Acad.Sci.USA, 99:14758-14763, 2002 Cited by PubMed Abstract: Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of trimeric capsomers. Each capsomer is composed of three molecules of the major capsid protein, Vp54, the 2.0-A resolution structure of which is reported here. Four N-linked and two O-linked glycosylation sites were identified. The N-linked sites are associated with nonstandard amino acid motifs as a result of glycosylation by virus-encoded enzymes. Each monomer of the trimeric structure consists of two eight-stranded, antiparallel beta-barrel, "jelly-roll" domains related by a pseudo-sixfold rotation. The fold of the monomer and the pseudo-sixfold symmetry of the capsomer resembles that of the major coat proteins in the double-stranded DNA bacteriophage PRD1 and the double-stranded DNA human adenoviruses, as well as the viral proteins VP2-VP3 of picornaviruses. The structural similarities among these diverse groups of viruses, whose hosts include bacteria, unicellular eukaryotes, plants, and mammals, make it probable that their capsid proteins have evolved from a common ancestor that had already acquired a pseudo-sixfold organization. The trimeric capsid protein structure was used to produce a quasi-atomic model of the 1,900-A diameter PBCV-1 outer shell, based on fitting of the Vp54 crystal structure into a three-dimensional cryoelectron microscopy image reconstruction of the virus. PubMed: 12411581DOI: 10.1073/pnas.232580699 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (28 Å) |
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