1M4L

STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION

1M4L の概要

• エントリーDOI: 10.2210/pdb1m4l/pdb
• 関連するPDBエントリー: 1YME 2CTB 5CPA
• 分子名称: CARBOXYPEPTIDASE A, ZINC ION (3 entities in total)
• 機能のキーワード: carboxypeptidase a, metalloproteinase, metalloexoproteinase, hydrolase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted, extracellular space: P00730
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34510.83

構造登録者

Kilshtain-Vardi, A.,Glick, M.,Greenblatt, H.M.,Goldblum, A.,Shoham, G. (登録日: 2002-07-03, 公開日: 2003-01-10, 最終更新日: 2024-10-30)

主引用文献

Kilshtain-Vardi, A.,Glick, M.,Greenblatt, H.M.,Goldblum, A.,Shoham, G.
Refined structure of bovine carboxypeptidase A at 1.25 A resolution.
Acta Crystallogr.,Sect.D, 59:323-333, 2003

PubMed Abstract: The crystal structure of the bovine zinc metalloproteinase carboxypeptidase A (CPA) has been refined to 1.25 A resolution based on room-temperature X-ray synchrotron data. The significantly improved structure of CPA at this resolution (anisotropic temperature factors, R factor = 10.4%, R(free) = 14.5%) allowed the modelling of conformational disorders of side chains, improved the description of the protein solvent network (375 water molecules) and provided a more accurate picture of the interactions between the active-site zinc and its ligands. The calculation of standard uncertainties in individual atom positions of the refined model of CPA allowed the deduction of the protonation state of some key residues in the active site and confirmed that Glu72 and Glu270 are negatively charged in the resting state of the enzyme at pH 7.5. These results were further validated by theoretical calculations that showed significant reduction of the pK(a) of these side chains relative to solution values. The distance between the zinc-bound solvent molecule and the metal ion is strongly suggestive of a neutral water molecule and not a hydroxide ion in the resting state of the enzyme. These findings could support both the general acid/general base mechanism, as well as the anhydride mechanism suggested for CPA.

PubMed: 12554943
DOI: 10.1107/S0907444902015706

実験手法

X-RAY DIFFRACTION (1.25 Å)