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1M4F

Solution Structure of Hepcidin-25

1M4F の概要
エントリーDOI10.2210/pdb1m4f/pdb
関連するPDBエントリー1m4e
NMR情報BMRB: 5501
分子名称Hepcidin (1 entity in total)
機能のキーワードstrand-loop-strand, beta-sheet, hairpin loop, antimicrobial protein
細胞内の位置Secreted: P81172
タンパク質・核酸の鎖数1
化学式量合計2802.45
構造登録者
Hunter, H.N.,Fulton, D.B.,Ganz, T.,Vogel, H.J. (登録日: 2002-07-02, 公開日: 2002-11-06, 最終更新日: 2024-10-16)
主引用文献Hunter, H.N.,Fulton, D.B.,Ganz, T.,Vogel, H.J.
The solution structure of human hepcidin, a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis.
J.Biol.Chem., 277:37597-37603, 2002
Cited by
PubMed Abstract: The antibacterial and antifungal peptide hepcidin (LEAP-1) is expressed in the liver. This circulating peptide has recently been found to also act as a signaling molecule in iron metabolism. As such, it plays an important role in hereditary hemochromatosis, a serious iron overload disease. In this study, we report the solution structures of the hepcidin-20 and -25 amino acid peptides determined by standard two-dimensional (1)H NMR spectroscopy. These small cysteine-rich peptides form a distorted beta-sheet with an unusual vicinal disulfide bridge found at the turn of the hairpin, which is probably of functional significance. Both peptides exhibit an overall amphipathic structure with six of the eight Cys involved in maintaining interstrand connectivity. Hepcidin-25 assumes major and minor conformations centered about the Pro residue near the N-terminal end. Further NMR diffusion studies indicate that hepcidin-20 exists as a monomer in solution, whereas hepcidin-25 readily aggregates, a property that may contribute to the different activities of the two peptides. The nuclear Overhauser enhancement spectroscopy spectra of the hepcidin-25 aggregates indicate an interface for peptide interactions that again involves the first five residues from the N-terminal end.
PubMed: 12138110
DOI: 10.1074/jbc.M205305200
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 1m4f
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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