1M2F

Solution structure of the N-terminal domain of Synechococcus elongatus KaiA (KaiA135N); Family of 25 structures

1M2F の概要

• エントリーDOI: 10.2210/pdb1m2f/pdb
• 関連するPDBエントリー: 1m2e
• NMR情報: BMRB: 5031
• 分子名称: KaiA (1 entity in total)
• 機能のキーワード: alpha-beta-alpha sandwich, circadian clock protein
• 由来する生物種: Synechococcus elongatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15077.12

構造登録者

Williams, S.B.,Vakonakis, I.,Golden, S.S.,LiWang, A.C. (登録日: 2002-06-23, 公開日: 2002-11-13, 最終更新日: 2024-05-22)

主引用文献

Williams, S.B.,Vakonakis, I.,Golden, S.S.,LiWang, A.C.
Structure and Function from the Circadian Clock Protein KaiA of Synechococcus elongatus: A potential Clock Input Mechanism
Proc.Natl.Acad.Sci.USA, 99:15357-15362, 2002

PubMed Abstract: In the cyanobacterium Synechococcus elongatus (PCC 7942) the proteins KaiA, KaiB, and KaiC are required for circadian clock function. We deduced a circadian clock function for KaiA from a combination of biochemical and structural data. Both KaiA and its isolated carboxyl-terminal domain (KaiA180C) stimulated KaiC autophosphorylation and facilitated attenuation of KaiC autophosphorylation by KaiB. An amino-terminal domain (KaiA135N) had no function in the autophosphorylation assay. NMR structure determination showed that KaiA135N is a pseudo-receiver domain. We propose that this pseudo-receiver is a timing input-device that regulates KaiA stimulation of KaiC autophosphorylation, which in turn is essential for circadian timekeeping.

PubMed: 12438647
DOI: 10.1073/pnas.232517099

実験手法

SOLUTION NMR