1M2C

THREE-DIMENSIONAL STRUCTURE OF ALPHA-CONOTOXIN MII, NMR, 14 STRUCTURES

1M2C の概要

• エントリーDOI: 10.2210/pdb1m2c/pdb
• 分子名称: ALPHA-CONOTOXIN MII (1 entity in total)
• 機能のキーワード: neurotoxin, neuronal nicotinic acetylcholine receptor inhibitor, subtype specific ligand, presynaptic nicotinic acetylcholine receptor blocker, cholinergic modulation, dopamine release
• 由来する生物種: Conus magus (magus cone)
• 細胞内の位置: Secreted: P56636
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1715.98

構造登録者

Shon, K.J.,Koerber, S.C.,Rivier, J.E.,Olivera, B.M.,Mcintosh, J.M. (登録日: 1997-09-15, 公開日: 1998-12-09, 最終更新日: 2024-10-30)

主引用文献

Cartier, G.E.,Yoshikami, D.,Gray, W.R.,Luo, S.,Olivera, B.M.,McIntosh, J.M.
A new alpha-conotoxin which targets alpha3beta2 nicotinic acetylcholine receptors.
J.Biol.Chem., 271:7522-7528, 1996

PubMed Abstract: We have isolated a 16-amino acid peptide from the venom of the marine snail Conus magus which potently blocks nicotinic acetylcholine receptors (nAChRs) composed of alpha3beta2 subunits. This peptide, named alpha-conotoxin MII, was identified by electrophysiologically screening venom fractions against cloned nicotinic receptors expressed in Xenopus oocytes. The peptide's structure, which has been confirmed by mass spectrometry and total chemical synthesis, differs significantly from those of all previously isolated alpha-conotoxins. Disulfide bridging, however, is conserved. The toxin blocks the response to acetylcholine in oocytes expressing alpha3beta2 nAChRs with an IC50 of 0.5 nM and is 2-4 orders of magnitude less potent on other nAChR subunit combinations. We have recently reported the isolation and characterization of alpha-conotoxin ImI, which selectively targets homomeric alpha7 neuronal nAChRs. Yet other alpha-conotoxins selectively block the muscle subtype of nAChR. Thus, it is increasingly apparent that alpha-conotoxins represent a significant resource for ligands with which to probe structure-function relationships of various nAChR subtypes.

PubMed: 8631783
DOI: 10.1074/jbc.271.13.7522

実験手法

SOLUTION NMR