1M0J

solution structure of the beta domain of mt_nc

1M0J の概要

• エントリーDOI: 10.2210/pdb1m0j/pdb
• 関連するPDBエントリー: 1M0G
• 分子名称: metallothionein MT_nc, CADMIUM ION (2 entities in total)
• 機能のキーワード: cadmium thiolate-cluster, metal binding protein
• 由来する生物種: Notothenia coriiceps (yellowbelly rockcod)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3150.45

構造登録者

Capasso, C.,Carginale, V.,Crescenzi, O.,Di Maro, D.,Parisi, E.,Spadaccini, R.,Temussi, P.A. (登録日: 2002-06-13, 公開日: 2003-05-06, 最終更新日: 2024-05-29)

主引用文献

Capasso, C.,Carginale, V.,Crescenzi, O.,Di Maro, D.,Parisi, E.,Spadaccini, R.,Temussi, P.A.
Solution Structure of MT_nc, a Novel Metallothionein from the Antarctic Fish Notothenia coriiceps.
Structure, 11:435-443, 2003

PubMed Abstract: The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins.

PubMed: 12679021
DOI: 10.1016/S0969-2126(03)00044-3

実験手法

SOLUTION NMR