1LU3

Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome

1LU3 の概要

• エントリーDOI: 10.2210/pdb1lu3/pdb
• 関連するPDBエントリー: 1EFC 1EXM 1LS2 1TTT
• EMDBエントリー: 1045
• 分子名称: PHENYLALANINE TRANSFER RNA (1 entity in total)
• 機能のキーワード: trna, ternary complex, cryo-em, 70s e.coli ribosome, conformational change, rna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5466.33

構造登録者

Valle, M.,Sengupta, J.,Swami, N.K.,Grassucci, R.A.,Burkhardt, N.,Nierhaus, K.H.,Agrawal, R.K.,Frank, J. (登録日: 2002-05-21, 公開日: 2002-06-26, 最終更新日: 2024-02-14)

主引用文献

Valle, M.,Sengupta, J.,Swami, N.K.,Grassucci, R.A.,Burkhardt, N.,Nierhaus, K.H.,Agrawal, R.K.,Frank, J.
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process.
EMBO J., 21:3557-3567, 2002

PubMed Abstract: During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.

PubMed: 12093756
DOI: 10.1093/emboj/cdf326

実験手法

ELECTRON MICROSCOPY (16.8 Å)