1LPE

THREE-DIMENSIONAL STRUCTURE OF THE LDL RECEPTOR-BINDING DOMAIN OF HUMAN APOLIPOPROTEIN E

1LPE の概要

• エントリーDOI: 10.2210/pdb1lpe/pdb
• 分子名称: APOLIPOPROTEIN E3 (2 entities in total)
• 機能のキーワード: lipoprotein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P02649
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16743.08

構造登録者

Wilson, C.,Agard, D.A. (登録日: 1991-08-22, 公開日: 1992-10-15, 最終更新日: 2024-02-14)

主引用文献

Wilson, C.,Wardell, M.R.,Weisgraber, K.H.,Mahley, R.W.,Agard, D.A.
Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E.
Science, 252:1817-1822, 1991

PubMed Abstract: Human apolipoprotein E, a blood plasma protein, mediates the transport and uptake of cholesterol and lipid by way of its high affinity interaction with different cellular receptors, including the low-density lipoprotein (LDL) receptor. The three-dimensional structure of the LDL receptor-binding domain of apoE has been determined at 2.5 angstrom resolution by x-ray crystallography. The protein forms an unusually elongated (65 angstroms) four-helix bundle, with the helices apparently stabilized by a tightly packed hydrophobic core that includes leucine zipper-type interactions and by numerous salt bridges on the mostly charged surface. Basic amino acids important for LDL receptor binding are clustered into a surface patch on one long helix. This structure provides the basis for understanding the behavior of naturally occurring mutants that can lead to atherosclerosis.

PubMed: 2063194

実験手法

X-RAY DIFFRACTION (2.25 Å)