1LOF

X-RAY STRUCTURE OF A BIANTENNARY OCTASACCHARIDE-LECTIN COMPLEX AT 2.3 ANGSTROMS RESOLUTION

Summary for 1LOF

• Entry DOI: 10.2210/pdb1lof/pdb
• Descriptor: LEGUME ISOLECTIN I (ALPHA CHAIN), LEGUME ISOLECTIN I (BETA CHAIN), beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: lectin
• Biological source: Lathyrus ochrus (yellow-flowered pea); More
• Total number of polymer chains: 4
• Total formula weight: 53521.30

Authors

Bourne, Y.,Cambillau, C. (deposition date: 1993-01-27, release date: 1994-04-30, Last modification date: 2024-02-14)

Primary citation

Bourne, Y.,Rouge, P.,Cambillau, C.
X-ray structure of a biantennary octasaccharide-lectin complex refined at 2.3-A resolution.
J.Biol.Chem., 267:197-203, 1992

PubMed Abstract: The structure and flexibility of saccharides have a profound and specific influence in several biological processes such as protein protection and the maintenance of conformational integrity, and in recognition events involving viruses, enzymes, and lectins. To establish the structural bases of these phenomena, we describe herein the extensively refined 2.3-A resolution x-ray structure of a biantennary octasaccharide of the N-acetyllactosamine type, complexed to isolectin I from Lathyrus ochrus. The two octasaccharides are located in clefts at each end of the long axis of the lectin. The conformations of both the lectin and the saccharide are slightly modified upon binding. The complex is stabilized by numerous hydrogen bonds, many of them involving water molecules. It is also stabilized by van der Waals interactions, including some with aromatic residues. A more general model of a possible lectin-glycoprotein interaction is also proposed.

PubMed: 1730588

Experimental method

X-RAY DIFFRACTION (2.3 Å)