1LOF

X-RAY STRUCTURE OF A BIANTENNARY OCTASACCHARIDE-LECTIN COMPLEX AT 2.3 ANGSTROMS RESOLUTION

1LOF の概要

• エントリーDOI: 10.2210/pdb1lof/pdb
• 分子名称: LEGUME ISOLECTIN I (ALPHA CHAIN), LEGUME ISOLECTIN I (BETA CHAIN), beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: lectin
• 由来する生物種: Lathyrus ochrus (yellow-flowered pea); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53521.30

構造登録者

Bourne, Y.,Cambillau, C. (登録日: 1993-01-27, 公開日: 1994-04-30, 最終更新日: 2024-02-14)

主引用文献

Bourne, Y.,Rouge, P.,Cambillau, C.
X-ray structure of a biantennary octasaccharide-lectin complex refined at 2.3-A resolution.
J.Biol.Chem., 267:197-203, 1992

PubMed Abstract: The structure and flexibility of saccharides have a profound and specific influence in several biological processes such as protein protection and the maintenance of conformational integrity, and in recognition events involving viruses, enzymes, and lectins. To establish the structural bases of these phenomena, we describe herein the extensively refined 2.3-A resolution x-ray structure of a biantennary octasaccharide of the N-acetyllactosamine type, complexed to isolectin I from Lathyrus ochrus. The two octasaccharides are located in clefts at each end of the long axis of the lectin. The conformations of both the lectin and the saccharide are slightly modified upon binding. The complex is stabilized by numerous hydrogen bonds, many of them involving water molecules. It is also stabilized by van der Waals interactions, including some with aromatic residues. A more general model of a possible lectin-glycoprotein interaction is also proposed.

PubMed: 1730588

実験手法

X-RAY DIFFRACTION (2.3 Å)