1LKD

CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2',6'-DICL DIHYDROXYBIPHENYL (DHB)

1LKD の概要

• エントリーDOI: 10.2210/pdb1lkd/pdb
• 関連するPDBエントリー: 1HAN 1KMY 1KND 1KNF 1LGT
• 分子名称: BIPHENYL-2,3-DIOL 1,2-DIOXYGENASE, FE (II) ION, 2',6'-DICHLORO-BIPHENYL-2,6-DIOL, ... (5 entities in total)
• 機能のキーワード: extradiol dioxygenase, 2, 3-dihydroxybiphenyl, non-heme iron, anaerobic, pcb biodegradation, oxidoreductase
• 由来する生物種: Burkholderia xenovorans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33281.81

構造登録者

Dai, S.,Bolin, J.T. (登録日: 2002-04-24, 公開日: 2002-11-27, 最終更新日: 2024-02-14)

主引用文献

Dai, S.,Vaillancourt, F.H.,Maaroufi, H.,Drouin, N.M.,Neau, D.B.,Snieckus, V.,Bolin, J.T.,Eltis, L.D.
Identification and analysis of a bottleneck in PCB biodegradation
Nat.Struct.Biol., 9:934-939, 2002

PubMed Abstract: The microbial degradation of polychlorinated biphenyls (PCBs) provides the potential to destroy these widespread, toxic and persistent environmental pollutants. For example, the four-step upper bph pathway transforms some of the more than 100 different PCBs found in commercial mixtures and is being engineered for more effective PCB degradation. In the critical third step of this pathway, 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD; EC 1.13.11.39) catalyzes aromatic ring cleavage. Here we demonstrate that ortho-chlorinated PCB metabolites strongly inhibit DHBD, promote its suicide inactivation and interfere with the degradation of other compounds. For example, k(cat)(app) for 2',6'-diCl DHB was reduced by a factor of approximately 7,000 relative to DHB, and it bound with sufficient affinity to competitively inhibit DHB cleavage at nanomolar concentrations. Crystal structures of two complexes of DHBD with ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation for these phenomena, which have important implications for bioremediation strategies.

PubMed: 12415290
DOI: 10.1038/nsb866

実験手法

X-RAY DIFFRACTION (1.7 Å)