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1LKD

CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2',6'-DICL DIHYDROXYBIPHENYL (DHB)

1LKD の概要
エントリーDOI10.2210/pdb1lkd/pdb
関連するPDBエントリー1HAN 1KMY 1KND 1KNF 1LGT
分子名称BIPHENYL-2,3-DIOL 1,2-DIOXYGENASE, FE (II) ION, 2',6'-DICHLORO-BIPHENYL-2,6-DIOL, ... (5 entities in total)
機能のキーワードextradiol dioxygenase, 2, 3-dihydroxybiphenyl, non-heme iron, anaerobic, pcb biodegradation, oxidoreductase
由来する生物種Burkholderia xenovorans
タンパク質・核酸の鎖数1
化学式量合計33281.81
構造登録者
Dai, S.,Bolin, J.T. (登録日: 2002-04-24, 公開日: 2002-11-27, 最終更新日: 2024-02-14)
主引用文献Dai, S.,Vaillancourt, F.H.,Maaroufi, H.,Drouin, N.M.,Neau, D.B.,Snieckus, V.,Bolin, J.T.,Eltis, L.D.
Identification and analysis of a bottleneck in PCB biodegradation
Nat.Struct.Biol., 9:934-939, 2002
Cited by
PubMed Abstract: The microbial degradation of polychlorinated biphenyls (PCBs) provides the potential to destroy these widespread, toxic and persistent environmental pollutants. For example, the four-step upper bph pathway transforms some of the more than 100 different PCBs found in commercial mixtures and is being engineered for more effective PCB degradation. In the critical third step of this pathway, 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD; EC 1.13.11.39) catalyzes aromatic ring cleavage. Here we demonstrate that ortho-chlorinated PCB metabolites strongly inhibit DHBD, promote its suicide inactivation and interfere with the degradation of other compounds. For example, k(cat)(app) for 2',6'-diCl DHB was reduced by a factor of approximately 7,000 relative to DHB, and it bound with sufficient affinity to competitively inhibit DHB cleavage at nanomolar concentrations. Crystal structures of two complexes of DHBD with ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation for these phenomena, which have important implications for bioremediation strategies.
PubMed: 12415290
DOI: 10.1038/nsb866
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.7 Å)
構造検証レポート
Validation report summary of 1lkd
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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