1LKB

Porcine Pancreatic Elastase/Na-Complex

Summary for 1LKB

• Entry DOI: 10.2210/pdb1lkb/pdb
• Related: 1LKA
• Descriptor: Elastase 1, SULFATE ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: chymotrypsin-fold, beta-barrel, sodium binding, chloride binding, sulfate binding, hydrolase
• Biological source: Sus scrofa (pig)
• Cellular location: Secreted: P00772
• Total number of polymer chains: 1
• Total formula weight: 26179.59

Authors

Weiss, M.S.,Panjikar, S.,Nowak, E.,Tucker, P.A. (deposition date: 2002-04-24, release date: 2002-08-28, Last modification date: 2024-10-16)

Primary citation

Weiss, M.S.,Panjikar, S.,Nowak, E.,Tucker, P.A.
Metal binding to porcine pancreatic elastase: calcium or not calcium.
Acta Crystallogr.,Sect.D, 58:1407-1412, 2002

PubMed Abstract: Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.

PubMed: 12198296
DOI: 10.1107/S0907444902010934

Experimental method

X-RAY DIFFRACTION (1.7 Å)