1LKA

Porcine Pancreatic Elastase/Ca-Complex

1LKA の概要

• エントリーDOI: 10.2210/pdb1lka/pdb
• 関連するPDBエントリー: 1LKB
• 分子名称: Elastase 1, ACETATE ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: chymotrypsin-fold, beta-barrel, calcium binding, chloride binding, acetate binding, hydrolase
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Secreted: P00772
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26063.59

構造登録者

Weiss, M.S.,Panjikar, S.,Nowak, E.,Tucker, P.A. (登録日: 2002-04-24, 公開日: 2002-08-28, 最終更新日: 2024-10-30)

主引用文献

Weiss, M.S.,Panjikar, S.,Nowak, E.,Tucker, P.A.
Metal binding to porcine pancreatic elastase: calcium or not calcium.
Acta Crystallogr.,Sect.D, 58:1407-1412, 2002

PubMed Abstract: Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.

PubMed: 12198296
DOI: 10.1107/S0907444902010934

実験手法

X-RAY DIFFRACTION (1.7 Å)