1LJZ

NMR structure of an AChR-peptide (Torpedo Californica, alpha-subunit residues 182-202) in complex with alpha-Bungarotoxin

Summary for 1LJZ

• Entry DOI: 10.2210/pdb1ljz/pdb
• Related: 1l4w
• NMR Information: BMRB: 4838
• Descriptor: alpha-Bungarotoxin, Acetylcholine receptor protein (2 entities in total)
• Functional Keywords: bungarotoxin, acetylcholine receptor, beta-hairpin, intermolecular beta-sheet, receptor, toxin
• Biological source: Bungarus multicinctus (many-banded krait); More
• Cellular location: Secreted: P60615; Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein: P02711
• Total number of polymer chains: 2
• Total formula weight: 11142.71

Authors

Samson, A.O.,Scherf, T.,Eisenstein, M.,Chill, J.H.,Anglister, J. (deposition date: 2002-04-23, release date: 2002-07-17, Last modification date: 2013-07-24)

Primary citation

Samson, A.,Scherf, T.,Eisenstein, M.,Chill, J.,Anglister, J.
The mechanism for acetylcholine receptor inhibition by alpha-neurotoxins and species-specific resistance to alpha-bungarotoxin revealed by NMR.
Neuron, 35:319-332, 2002

PubMed Abstract: The structure of a peptide corresponding to residues 182-202 of the acetylcholine receptor alpha1 subunit in complex with alpha-bungarotoxin was solved using NMR spectroscopy. The peptide contains the complete sequence of the major determinant of AChR involved in alpha-bungarotoxin binding. One face of the long beta hairpin formed by the AChR peptide consists of exposed nonconserved residues, which interact extensively with the toxin. Mutations of these receptor residues confer resistance to the toxin. Conserved AChR residues form the opposite face of the beta hairpin, which creates the inner and partially hidden pocket for acetylcholine. An NMR-derived model for the receptor complex with two alpha-bungarotoxin molecules shows that this pocket is occupied by the conserved alpha-neurotoxin residue R36, which forms cation-pi interactions with both alphaW149 and gammaW55/deltaW57 of the receptor and mimics acetylcholine.

PubMed: 12160749
DOI: 10.1016/S0896-6273(02)00773-0

Experimental method

SOLUTION NMR