1LFQ

OXY HEMOGLOBIN (93% RELATIVE HUMIDITY)

1LFQ の概要

• エントリーDOI: 10.2210/pdb1lfq/pdb
• 関連するPDBエントリー: 1LFL 1LFT 1LFV 1LFY 1LFZ
• 分子名称: Hemoglobin alpha chain, Hemoglobin beta chain, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: hemoglobin, t state, humidity, environment, oxygen storage-transport complex, oxygen storage/transport
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32273.52

構造登録者

Biswal, B.K.,Vijayan, M. (登録日: 2002-04-12, 公開日: 2002-10-12, 最終更新日: 2024-02-14)

主引用文献

Biswal, B.K.,Vijayan, M.
Structures of human oxy- and deoxyhaemoglobin at different levels of humidity: variability in the T state.
Acta Crystallogr.,Sect.D, 58:1155-1161, 2002

PubMed Abstract: High-salt crystals of human oxy- and deoxyhaemoglobin have been studied at different levels of environmental humidity and solvent content. The structure of the oxy form remains relatively unchanged at all levels. The deoxy form, however, undergoes a water-mediated transformation when the relative humidity around the crystals is reduced below 93%. The space group is maintained during the transformation, but the unit-cell volume nearly doubles, with two tetrameric molecules in the asymmetric unit of the low-humidity form compared with one in the native crystals. Interestingly, the haem geometry in the low-humidity form is closer to that in the oxy form than to that in the native deoxy form. The quaternary structure of one of the tetramers moves slightly towards that in the oxy form, while that in the other is more different from the oxy form than that in the high-salt native deoxy form. Thus, it would appear that, as in the case of the liganded form, the deoxy form of haemoglobin can also access an ensemble of related T states.

PubMed: 12077435
DOI: 10.1107/S0907444902007138

実験手法

X-RAY DIFFRACTION (2.6 Å)