1LES

LENTIL LECTIN COMPLEXED WITH SUCROSE

Summary for 1LES

• Entry DOI: 10.2210/pdb1les/pdb
• Related PRD ID: PRD_900003
• Descriptor: LENTIL LECTIN, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: protein-sugar interactions, lectin
• Biological source: Lens culinaris (lentil); More
• Total number of polymer chains: 4
• Total formula weight: 52185.29

Authors

Hamelryck, T.,Loris, R. (deposition date: 1995-08-23, release date: 1995-12-07, Last modification date: 2024-02-14)

Primary citation

Casset, F.,Hamelryck, T.,Loris, R.,Brisson, J.R.,Tellier, C.,Dao-Thi, M.H.,Wyns, L.,Poortmans, F.,Perez, S.,Imberty, A.
NMR, molecular modeling, and crystallographic studies of lentil lectin-sucrose interaction.
J.Biol.Chem., 270:25619-25628, 1995

PubMed Abstract: The conformational features of sucrose in the combining site of lentil lectin have been characterized through elucidation of a crystalline complex at 1.9-A resolution, transferred nuclear Overhauser effect experiments performed at 600 Mhz, and molecular modeling. In the crystal, the lentil lectin dimer binds one sucrose molecule per monomer. The locations of 229 water molecules have been identified. NMR experiments have provided 11 transferred NOEs. In parallel, the docking study and conformational analysis of sucrose in the combining site of lentil lectin indicate that three different conformations can be accommodated. Of these, the orientation with lowest energy is identical with the one observed in the crystalline complex and provides good agreement with the observed transferred NOEs. These structural investigations indicate that the bound sucrose has a unique conformation for the glycosidic linkage, close to the one observed in crystalline sucrose, whereas the fructofuranose ring remains relatively flexible and does not exhibit any strong interaction with the protein. Major differences in the hydrogen bonding network of sucrose are found. None of the two inter-residue hydrogen bonds in crystalline sucrose are conserved in the complex with the lectin. Instead, a water molecule bridges hydroxyl groups O2-g and O3-f of sucrose.

PubMed: 7592736
DOI: 10.1074/jbc.270.43.25619

Experimental method

X-RAY DIFFRACTION (1.9 Å)