1LE7

CARBOXYLIC ESTER HYDROLASE, C 2 2 21 space group

1LE7 の概要

• エントリーDOI: 10.2210/pdb1le7/pdb
• 関連するPDBエントリー: 1LE6
• 分子名称: Group X Secretory Phospholipase A2, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: human phosphatidylcholine 2-acylhydrolase gx, gx spla2, spla2-x, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: O15496
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27960.10

構造登録者

Pan, Y.H.,Jain, M.K.,Bahnson, B.J. (登録日: 2002-04-09, 公開日: 2002-08-14, 最終更新日: 2024-10-30)

主引用文献

Pan, Y.H.,Yu, B.Z.,Singer, A.G.,Ghomashchi, F.,Lambeau, G.,Gelb, M.H.,Jain, M.K.,Bahnson, B.J.
Crystal structure of human group X secreted phospholipase A2. Electrostatically neutral interfacial surface targets zwitterionic membranes.
J.Biol.Chem., 277:29086-29093, 2002

PubMed Abstract: The crystal structure of human group X (hGX) secreted phospholipase A2 (sPLA2) has been solved to a resolution of 1.97 A. As expected the protein fold is similar to previously reported sPLA2 structures. The active site architecture, including the positions of the catalytic residues and the first and second shell water around the Ca2+ cofactor, are highly conserved and remarkably similar to the group IB and group IIA enzymes. Differences are seen in the structures following the (1-12)-N-terminal helix and at the C terminus. These regions are proposed to interact with the substrate membrane surface. The opening to the active site slot is considerably larger in hGX than in human group IIA sPLA2. Furthermore, the electrostatic surface potential of the hGX interfacial-binding surface does not resemble that of the human group IIA sPLA2; the former is highly neutral, whereas the latter is highly cationic. The cationic residues on this face of group IB and IIA enzymes have been implicated in membrane binding and in k(cat*) allostery. In contrast, hGX does not show activation by the anionic charge at the lipid interface when acting on phospholipid vesicles or short-chain phospholipid micelles. Together, the crystal structure and kinetic results of hGX supports the conclusion that it is as active on zwitterionic as on anionic interfaces, and thus it is predicted to target the zwitterionic membrane surfaces of mammalian cells.

PubMed: 12161451
DOI: 10.1074/jbc.M202531200

実験手法

X-RAY DIFFRACTION (2.09 Å)