1LC2

Solution Structure Of Reduced Horse Heart Cytochrome c in 30% Acetonitrile Solution, NMR 30 Structures

1LC2 の概要

• エントリーDOI: 10.2210/pdb1lc2/pdb
• 関連するPDBエントリー: 1LC1
• NMR情報: BMRB: 5372
• 分子名称: CYTOCHROME C, HEME C (2 entities in total)
• 機能のキーワード: cytochrome c, organic solvent, electron transport
• 由来する生物種: Equus caballus (horse)
• 細胞内の位置: Mitochondrion matrix: P00004
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12344.10

構造登録者

Sivakolundu, S.G.,Mabrouk, P.A. (登録日: 2002-04-04, 公開日: 2003-06-03, 最終更新日: 2024-10-30)

主引用文献

Sivakolundu, S.G.,Mabrouk, P.A.
Structure function relationship of reduced cytochrome c probed by complete solution structure determination in 30% acetonitrile/water solution
J.BIOL.INORG.CHEM., 8:527-539, 2003

PubMed Abstract: The complete solution structure of ferrocytochrome c in 30% acetonitrile/70% water has been determined using high-field 1D and 2D (1)H NMR methods and deposited in the Protein Data Bank with codes 1LC1 and 1LC2. This is the first time a complete solution protein structure has been determined for a protein in nonaqueous media. Ferrocyt c retains a native protein secondary structure (five alpha-helices and two omega loops) in 30% acetonitrile. H18 and M80 residues are the axial heme ligands, as in aqueous solution. Residues believed to be axial heme ligands in the alkaline-like conformers of ferricyt c, specifically H33 and K72, are positioned close to the heme iron. The orientations of both heme propionates are markedly different in 30% acetonitrile/70% water. Comparative structural analysis of reduced cyt c in 30% acetonitrile/70% water solution with cyt c in different environments has given new insight into the cyt c folding mechanism, the electron transfer pathway, and cell apoptosis.

PubMed: 12764601

実験手法

SOLUTION NMR