1L8W

Crystal Structure of Lyme Disease Variable Surface Antigen VlsE of Borrelia burgdorferi

Summary for 1L8W

• Entry DOI: 10.2210/pdb1l8w/pdb
• Descriptor: VlsE1 (2 entities in total)
• Functional Keywords: variable surface protein, vmp-like sequence, immune system
• Biological source: Borreliella burgdorferi (Lyme disease spirochete)
• Cellular location: Cell outer membrane ; Lipid-anchor : O06878
• Total number of polymer chains: 4
• Total formula weight: 141097.06

Authors

Eicken, C.,Sharma, V.,Klabunde, T.,Lawrenz, M.B.,Hardham, J.M.,Norris, S.J.,Sacchettini, J.C. (deposition date: 2002-03-21, release date: 2002-06-19, Last modification date: 2018-03-07)

Primary citation

Eicken, C.,Sharma, V.,Klabunde, T.,Lawrenz, M.B.,Hardham, J.M.,Norris, S.J.,Sacchettini, J.C.
Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi.
J.Biol.Chem., 277:21691-21696, 2002

PubMed Abstract: VlsE is an outer surface lipoprotein of Borrelia burgdorferi that undergoes antigenic variation through an elaborate gene conversion mechanism and is thought to play a major role in the immune response to the Lyme disease borellia. The crystal structure of recombinant variant protein VlsE1 at 2.3-A resolution reveals that the six variable regions form loop structures that constitute most of the membrane distal surface of VlsE, covering the predominantly alpha-helical, invariant regions of the protein. The surface localization of the variable amino acid segments appears to protect the conserved regions from interaction with antibodies and hence may contribute to immune evasion.

PubMed: 11923306
DOI: 10.1074/jbc.M201547200

Experimental method

X-RAY DIFFRACTION (2.3 Å)