1L5I

30-CONFORMER NMR ENSEMBLE OF THE N-TERMINAL, DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN FROM A GEMINIVIRUS (TOMATO YELLOW LEAF CURL VIRUS-SARDINIA)

1L5I の概要

• エントリーDOI: 10.2210/pdb1l5i/pdb
• 関連するPDBエントリー: 1L2M
• NMR情報: BMRB: 5297
• 分子名称: Rep protein (1 entity in total)
• 機能のキーワード: a+b fold, rbd-like fold, viral protein
• 由来する生物種: Tomato yellow leaf curl Sardinia virus
• 細胞内の位置: Host nucleus (By similarity): P27260
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13583.30

構造登録者

Campos-Olivas, R.,Louis, J.M.,Clerot, D.,Gronenborn, B.,Gronenborn, A.M. (登録日: 2002-03-07, 公開日: 2002-09-18, 最終更新日: 2024-05-22)

主引用文献

Campos-Olivas, R.,Louis, J.M.,Clerot, D.,Gronenborn, B.,Gronenborn, A.M.
The structure of a replication initiator unites diverse aspects of nucleic acid metabolism
Proc.Natl.Acad.Sci.USA, 99:10310-10315, 2002

PubMed Abstract: Rolling circle replication is a mechanism for copying single-stranded genomes by means of double-stranded intermediates. A multifunctional replication initiator protein (Rep) is indispensable for the precise initiation and termination of this process. Despite the ubiquitous presence and fundamental importance of rolling circle replication elements, structural information on their respective replication initiators is still missing. Here we present the solution NMR structure of the catalytic domain of Rep, the initiator protein of tomato yellow leaf curl virus. It is composed of a central five-stranded anti-parallel beta-sheet, flanked by a small two-stranded beta-sheet, a beta-hairpin and two alpha-helices. Surprisingly, the structure reveals that the catalytic Rep domain is related to a large group of proteins that bind RNA or DNA. Identification of Rep as resembling the family of ribonucleoprotein/RNA-recognition motif fold proteins establishes a structure-based evolutionary link between RNA binding proteins, splicing factors, and replication initiators of prokaryotic and eukaryotic single-stranded DNA elements and mammalian DNA tumor viruses.

PubMed: 12130667
DOI: 10.1073/pnas.152342699

実験手法

SOLUTION NMR