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1L4T

SOLUTION NMR STRUCTURE OF THE CCK2E3

1L4T の概要
エントリーDOI10.2210/pdb1l4t/pdb
分子名称Gastrin/cholecystokinin type B receptor (1 entity in total)
機能のキーワードhormone/growth factor receptor, hormone-growth factor receptor complex
細胞内の位置Cell membrane; Multi-pass membrane protein: P32239
タンパク質・核酸の鎖数1
化学式量合計2991.39
構造登録者
Giragossian, C.,Mierke, D.F. (登録日: 2002-03-05, 公開日: 2003-01-28, 最終更新日: 2022-02-23)
主引用文献Giragossian, C.,Mierke, D.F.
Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin-2 receptor
Biochemistry, 41:4560-4566, 2002
Cited by
PubMed Abstract: The structure of the third extracellular loop of the human cholecystokinin-2 receptor, CCK2-R(352-379), and its interactions with the C-terminal octapeptide of cholecystokinin (CCK-8) have been determined by high-resolution NMR and computer simulations. In the presence of dodecylphosphocholine micelles, the structure of the receptor fragment consisted of three helices, with the first and third corresponding to residues of the extracellular ends of transmembrane helices (TM) 6 and 7, respectively. The central, extracellular helix, consisting of residues 363-368, was found to be closely associated with the membrane mimetic used during the spectroscopic studies and molecular dynamics (MD) simulations. Upon titration of CCK-8 to the receptor domain, chemical shift perturbation and intermolecular NOEs (Trp30, Met31 of CCK-8 and P371, F374 of CCK2-R) indicated the formation of a stable complex and specific ligand/receptor interactions. Using the NOE-generated intermolecular contact points, extensive MD simulations of CCK-8 bound to the CCK2 receptor were carried out. The results, with CCK-8 in close proximity to TM7, differ from previous structural studies of CCK-8 association with CCK1-R, in which the ligand formed a number of interactions with TM6. These differences may play a role in the ligand specificity displayed by the CCK1 and CCK2 receptor subtypes.
PubMed: 11926817
DOI: 10.1021/bi0160009
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実験手法
SOLUTION NMR
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件を2024-11-06に公開中

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