1L4A

X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI

1L4A の概要

• エントリーDOI: 10.2210/pdb1l4a/pdb
• 関連するPDBエントリー: 1KIL 1SFC
• 分子名称: SYNAPTOBREVIN, S-SYNTAXIN, S-SNAP25 fusion protein, ... (6 entities in total)
• 機能のキーワード: snare, snare complex, membrane fusion, neurotransmission, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Loligo pealei; 詳細
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P47194; Membrane; Lipid-anchor (Potential): Q95PA1
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 47517.12

構造登録者

Bracher, A.,Kadlec, J.,Betz, H.,Weissenhorn, W. (登録日: 2002-03-04, 公開日: 2002-07-31, 最終更新日: 2023-08-16)

主引用文献

Bracher, A.,Kadlec, J.,Betz, H.,Weissenhorn, W.
X-ray structure of a neuronal complexin-SNARE complex from squid.
J.Biol.Chem., 277:26517-26523, 2002

PubMed Abstract: Nerve terminals release neurotransmitters from vesicles into the synaptic cleft upon transient increases in intracellular Ca(2+). This exocytotic process requires the formation of trans SNARE complexes and is regulated by accessory proteins including the complexins. Here we report the crystal structure of a squid core complexin-SNARE complex at 2.95-A resolution. A helical segment of complexin binds in anti-parallel fashion to the four-helix bundle of the core SNARE complex and interacts at its C terminus with syntaxin and synaptobrevin around the ionic zero layer of the SNARE complex. We propose that this structure is part of a multiprotein fusion machinery that regulates vesicle fusion at a late pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by acting directly or indirectly on complexin, thus allowing the conformational transitions of the trans SNARE complex that are thought to drive membrane fusion.

PubMed: 12004067
DOI: 10.1074/jbc.M203460200

実験手法

X-RAY DIFFRACTION (2.95 Å)