1L3K

UP1, THE TWO RNA-RECOGNITION MOTIF DOMAIN OF HNRNP A1

1L3K の概要

• エントリーDOI: 10.2210/pdb1l3k/pdb
• 分子名称: HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 (2 entities in total)
• 機能のキーワード: nuclear protein hnrnp a1, rna-recognition motif, rna-binding, up1, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P09651
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22303.08

構造登録者

Vitali, J.,Ding, J.,Jiang, J.,Zhang, Y.,Krainer, A.R.,Xu, R.-M. (登録日: 2002-02-27, 公開日: 2002-04-17, 最終更新日: 2023-08-16)

主引用文献

Vitali, J.,Ding, J.,Jiang, J.,Zhang, Y.,Krainer, A.R.,Xu, R.M.
Correlated alternative side chain conformations in the RNA-recognition motif of heterogeneous nuclear ribonucleoprotein A1.
Nucleic Acids Res., 30:1531-1538, 2002

PubMed Abstract: The RNA-recognition motif (RRM) is a common and evolutionarily conserved RNA-binding module. Crystallographic and solution structural studies have shown that RRMs adopt a compact alpha/beta structure, in which four antiparallel beta-strands form the major RNA-binding surface. Conserved aromatic residues in the RRM are located on the surface of the beta-sheet and are important for RNA binding. To further our understanding of the structural basis of RRM-nucleic acid interaction, we carried out a high resolution analysis of UP1, the N-terminal, two-RRM domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), whose structure was previously solved at 1.75-1.9 A resolution. The two RRMs of hnRNP A1 are closely related but have distinct functions in regulating alternative pre-mRNA splice site selection. Our present 1.1 A resolution crystal structure reveals that two conserved solvent-exposed phenylalanines in the first RRM have alternative side chain conformations. These conformations are spatially correlated, as the individual amino acids cannot adopt each of the observed conformations independently. These phenylalanines are critical for nucleic acid binding and the observed alternative side chain conformations may serve as a mechanism for regulating nucleic acid binding by RRM-containing proteins.

PubMed: 11917013
DOI: 10.1093/nar/30.7.1531

実験手法

X-RAY DIFFRACTION (1.1 Å)