1L2Q

Crystal Structure of the Methanosarcina barkeri Monomethylamine Methyltransferase (MtmB)

1L2Q の概要

• エントリーDOI: 10.2210/pdb1l2q/pdb
• 関連するPDBエントリー: 1L2R
• 分子名称: monomethylamine methyltransferase, AMMONIUM ION (3 entities in total)
• 機能のキーワード: tim barrel, transferase
• 由来する生物種: Methanosarcina barkeri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50326.30

構造登録者

Hao, B.,Gong, W.,Ferguson, T.K.,James, C.M.,Krzycki, J.A.,Chan, M.K. (登録日: 2002-02-24, 公開日: 2002-06-05, 最終更新日: 2025-03-26)

主引用文献

Hao, B.,Gong, W.,Ferguson, T.K.,James, C.M.,Krzycki, J.A.,Chan, M.K.
A new UAG-encoded residue in the structure of a methanogen methyltransferase.
Science, 296:1462-1466, 2002

PubMed Abstract: Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.

PubMed: 12029132
DOI: 10.1126/science.1069556

実験手法

X-RAY DIFFRACTION (1.7 Å)