1L2H

Crystal structure of Interleukin 1-beta F42W/W120F mutant

1L2H の概要

• エントリーDOI: 10.2210/pdb1l2h/pdb
• 関連するPDBエントリー: 9ilb
• 分子名称: Interleukin 1-beta (2 entities in total)
• 機能のキーワード: interleukin-1 beta, beta-trefoil, f42w/w120f double mutant, protein folding, mad phasing, hemihedral twinning, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P01584
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17395.83

構造登録者

Rudolph, M.G.,Kelker, M.S.,Schneider, T.R.,Yeates, T.O.,Oseroff, V.,Heidary, D.K.,Jennings, P.A.,Wilson, I.A. (登録日: 2002-02-21, 公開日: 2003-02-04, 最終更新日: 2024-02-14)

主引用文献

Rudolph, M.G.,Kelker, M.S.,Schneider, T.R.,Yeates, T.O.,Oseroff, V.,Heidary, D.K.,Jennings, P.A.,Wilson, I.A.
Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta.
Acta Crystallogr.,Sect.D, 59:290-298, 2003

PubMed Abstract: The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data.

PubMed: 12554939
DOI: 10.1107/S0907444902021704

実験手法

X-RAY DIFFRACTION (1.54 Å)