1L2E
Human Kallikrein 6 (hK6) Active Form with benzamidine inhibitor
Summary for 1L2E
| Entry DOI | 10.2210/pdb1l2e/pdb |
| Descriptor | Kallikrein 6, MAGNESIUM ION, BENZAMIDINE, ... (4 entities in total) |
| Functional Keywords | serine protease, kallikrein, human kallikrein 6, benzamidine, protease, myelencephalon specific protease, zyme, protease m, neurosin, hydrolase |
| Biological source | Homo sapiens (human) |
| Cellular location | Secreted: Q92876 |
| Total number of polymer chains | 1 |
| Total formula weight | 24677.33 |
| Authors | Bernett, M.J.,Blaber, S.I.,Scarisbrick, I.A.,Blaber, M. (deposition date: 2002-02-20, release date: 2002-05-08, Last modification date: 2024-10-30) |
| Primary citation | Bernett, M.J.,Blaber, S.I.,Scarisbrick, I.A.,Dhanarajan, P.,Thompson, S.M.,Blaber, M. Crystal structure and biochemical characterization of human kallikrein 6 reveals that a trypsin-like kallikrein is expressed in the central nervous system. J.Biol.Chem., 277:24562-24570, 2002 Cited by PubMed Abstract: The human kallikreins are a large multigene family of closely related serine-type proteases. In this regard, they are similar to the multigene kallikrein families characterized in mice and rats. There is a much more extensive body of knowledge regarding the function of mouse and rat kallikreins in comparison with the human kallikreins. Human kallikrein 6 has been proposed as the homologue to rat myelencephalon-specific protease, an arginine-specific degradative-type protease abundantly expressed in the central nervous system and implicated in demyelinating disease. We present the x-ray crystal structure of mature, active recombinant human kallikrein 6 at 1.75-A resolution. This high resolution model provides the first three-dimensional view of one of the human kallikreins and one of only a few structures of serine proteases predominantly expressed in the central nervous system. Enzymatic data are presented that support the identification of human kallikrein 6 as the functional homologue of rat myelencephalon-specific protease and are corroborated by a molecular phylogenetic analysis. Furthermore, the x-ray data provide support for the characterization of human kallikrein 6 as a degradative protease with structural features more similar to trypsin than the regulatory kallikreins. PubMed: 11983703DOI: 10.1074/jbc.M202392200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.75 Å) |
Structure validation
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