1L2E

Human Kallikrein 6 (hK6) Active Form with benzamidine inhibitor

1L2E の概要

• エントリーDOI: 10.2210/pdb1l2e/pdb
• 分子名称: Kallikrein 6, MAGNESIUM ION, BENZAMIDINE, ... (4 entities in total)
• 機能のキーワード: serine protease, kallikrein, human kallikrein 6, benzamidine, protease, myelencephalon specific protease, zyme, protease m, neurosin, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: Q92876
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24677.33

構造登録者

Bernett, M.J.,Blaber, S.I.,Scarisbrick, I.A.,Blaber, M. (登録日: 2002-02-20, 公開日: 2002-05-08, 最終更新日: 2024-10-30)

主引用文献

Bernett, M.J.,Blaber, S.I.,Scarisbrick, I.A.,Dhanarajan, P.,Thompson, S.M.,Blaber, M.
Crystal structure and biochemical characterization of human kallikrein 6 reveals that a trypsin-like kallikrein is expressed in the central nervous system.
J.Biol.Chem., 277:24562-24570, 2002

PubMed Abstract: The human kallikreins are a large multigene family of closely related serine-type proteases. In this regard, they are similar to the multigene kallikrein families characterized in mice and rats. There is a much more extensive body of knowledge regarding the function of mouse and rat kallikreins in comparison with the human kallikreins. Human kallikrein 6 has been proposed as the homologue to rat myelencephalon-specific protease, an arginine-specific degradative-type protease abundantly expressed in the central nervous system and implicated in demyelinating disease. We present the x-ray crystal structure of mature, active recombinant human kallikrein 6 at 1.75-A resolution. This high resolution model provides the first three-dimensional view of one of the human kallikreins and one of only a few structures of serine proteases predominantly expressed in the central nervous system. Enzymatic data are presented that support the identification of human kallikrein 6 as the functional homologue of rat myelencephalon-specific protease and are corroborated by a molecular phylogenetic analysis. Furthermore, the x-ray data provide support for the characterization of human kallikrein 6 as a degradative protease with structural features more similar to trypsin than the regulatory kallikreins.

PubMed: 11983703
DOI: 10.1074/jbc.M202392200

実験手法

X-RAY DIFFRACTION (1.75 Å)