1L1Z

MutM (Fpg) Covalent-DNA Intermediate

1L1Z の概要

• エントリーDOI: 10.2210/pdb1l1z/pdb
• 関連するPDBエントリー: 1L1T 1L2B 1L2C 1L2D
• 分子名称: 5'-D(*AP*G*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3', 5'-D(*TP*GP*C*GP*TP*CP*CP*AP*(PED)P*GP*TP*CP*TP*AP*CP*C)-3', MutM, ... (5 entities in total)
• 機能のキーワード: dna repair, dna glycosylase, borohydride, zinc finger, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 40456.18

構造登録者

Fromme, J.C.,Verdine, G.L. (登録日: 2002-02-20, 公開日: 2002-06-14, 最終更新日: 2023-08-16)

主引用文献

Fromme, J.C.,Verdine, G.L.
Structural insights into lesion recognition and repair by the bacterial 8-oxoguanine DNA glycosylase MutM.
Nat.Struct.Biol., 9:544-552, 2002

PubMed Abstract: MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating base-excision repair of oxidized guanine residues in DNA. Here we report five different crystal structures of MutM-DNA complexes that represent different steps of the repair reaction cascade catalyzed by the protein and also differ in the identity of the base opposite the lesion (the 'estranged' base). These structures reveal that the MutM active site performs the multiple steps of base-excision and 3' and 5' nicking with minimal rearrangement of the DNA backbone.

PubMed: 12055620

実験手法

X-RAY DIFFRACTION (1.7 Å)