1L1W
NMR structure of a SRP19 binding domain in human SRP RNA
Summary for 1L1W
| Entry DOI | 10.2210/pdb1l1w/pdb |
| NMR Information | BMRB: 5321 |
| Descriptor | SRP19 binding domain of SRP RNA (1 entity in total) |
| Functional Keywords | rna hairpin, tetraloop, gnrr, mismatch, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 9407.69 |
| Authors | Sakamoto, T.,Morita, S.,Tabata, K.,Nakamura, K.,Kawai, G. (deposition date: 2002-02-20, release date: 2002-05-22, Last modification date: 2024-05-22) |
| Primary citation | Sakamoto, T.,Morita, S.,Tabata, K.,Nakamura, K.,Kawai, G. Solution structure of a SRP19 binding domain in human SRP RNA. J.Biochem.(Tokyo), 132:177-182, 2002 Cited by PubMed Abstract: Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop. PubMed: 12153712PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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