1L1W

NMR structure of a SRP19 binding domain in human SRP RNA

1L1W の概要

• エントリーDOI: 10.2210/pdb1l1w/pdb
• NMR情報: BMRB: 5321
• 分子名称: SRP19 binding domain of SRP RNA (1 entity in total)
• 機能のキーワード: rna hairpin, tetraloop, gnrr, mismatch, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9407.69

構造登録者

Sakamoto, T.,Morita, S.,Tabata, K.,Nakamura, K.,Kawai, G. (登録日: 2002-02-20, 公開日: 2002-05-22, 最終更新日: 2024-05-22)

主引用文献

Sakamoto, T.,Morita, S.,Tabata, K.,Nakamura, K.,Kawai, G.
Solution structure of a SRP19 binding domain in human SRP RNA.
J.Biochem.(Tokyo), 132:177-182, 2002

PubMed Abstract: Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop.

PubMed: 12153712

実験手法

SOLUTION NMR